I've been collecting sports cards and some TCG stuff (mostly Pokemon and Magic) since around 2012, and I've probably gone through 200+ different card storage solutions over the years. Started with penny sleeves and toploaders like everyone does, moved to magnetic holders for expensive cards, tried various acrylic cases, and eventually settled on a mixed system depending on the card value and how often I need to access it.
The physical protection part works fine
Acrylic boxes do prevent the most obvious types of damage-creasing, corner wear, edge chipping. A card in a proper acrylic case isn't going to get bent if you drop something on it or if it gets shuffled around in storage. I've had cards in Ultra Pro acrylic one-touch holders survive a move across three states with zero damage. Those same cards in toploaders probably would've been fine too, but the acrylic definitely adds peace of mind.
The thickness matters more than people realize. Cheap acrylic cases use 1.5mm or 2mm thick material and you can feel how flimsy they are. Better cases use 3mm or even 4mm acrylic which is substantially more rigid. I bought some no-name cases off Amazon for like $2.80 each (pack of 10, so $28 total) back in 2019 and they flexed noticeably when you squeezed them. The Ultra Pro cases at $4-5 each are way more solid.
There's also the fitment issue that nobody talks about enough. Standard trading cards are 2.5" × 3.5" but card thickness varies wildly. A base rookie card might be 20pt thick, but a patch autograph card could be 100pt or more. Most acrylic cases are designed for standard thickness cards and they don't grip thicker cards well. The card just slides around inside which defeats the purpose. You need magnetic holders or recessed cases for thick cards.

UV protection is where things get complicated
A lot of acrylic cases advertise UV protection but the specs are all over the place. Regular acrylic (PMMA) blocks some UV naturally-polymethyl methacrylate has decent UV resistance compared to glass. According to material data from Evonik (evonik.com), standard acrylic blocks about 92-95% of UV-B radiation but only around 70% of UV-A. That's not great if you're worried about long-term fading.
UV-resistant acrylic is a different formulation with additives that improve the blocking. The better cases claim 98-99% UV protection but I've never seen independent testing to verify those numbers. Pro-Mold cases mention UV filtering in their marketing materials but don't publish actual transmission data. BCW also makes UV-protected cases but again, no real specs beyond "blocks harmful UV rays" which tells you nothing.
I tested this myself in a completely unscientific way-put duplicate Pokemon cards (Charizard base set reprints, nothing valuable) in three different cases: cheap Amazon acrylic, Ultra Pro UV case, and Pro-Mold UV case. Left them on a windowsill that gets direct sun for about 6 hours a day. After 8 months the cheap case card had noticeable fading on the red/orange colors. The Ultra Pro and Pro-Mold cards looked identical to a control card kept in a dark drawer. So the UV protection does something, at least for visible light fading.
Humidity and temperature are the real enemies
Acrylic cases are not airtight. At all. They have seams where the two halves meet and air gets in freely. This means humidity control is entirely dependent on your storage environment, not the case itself. A card in an acrylic box stored in a humid basement is going to have moisture problems eventually.
The PSA registry shows condition census data for graded cards, and based on their population reports (psacard.com/pop), humidity damage is one of the most common condition issues for vintage cards. You see staining, warping, and mold growth on cards that were supposedly "stored safely" but were in environments with fluctuating humidity.
Ideal storage conditions are 40-50% relative humidity and 18-21°C temperature according to archival standards. Most people don't monitor this. I didn't for years until I had some older cards develop edge staining from what I assume was moisture absorption. Bought a $15 hygrometer from Amazon and found out my storage closet was running at 65-70% humidity in summer. That's way too high.
Silica gel packets help but they saturate after absorbing a certain amount of moisture. You're supposed to replace or recharge them (you can dry them out in an oven at 120°C for a few hours). Nobody does this. I have desiccant packets in my card storage boxes that have probably been there for 5+ years and are completely useless at this point.
Some higher-end storage solutions use desiccant holders that are meant to be maintained, like the ones from ProTech (protech-group.com, they make archival storage for museums and stuff). Those run $40-80+ per container though, which is impractical for most card collections.
The scratching issue on display cases
Acrylic scratches way easier than glass. This is fine for storage cases you're not handling constantly, but display cases that you're opening and closing regularly will show scratches within months. The magnetic closure cases are especially bad for this because the magnets trap dust particles that act as abrasives.
I have some higher-value cards in magnetic holders (Ultra Pro magnetics, the black border ones that run about $8-10 each) and after maybe 2 years of occasional opening to check the cards, the acrylic has fine scratches all over. Still functional, still protective, but it looks worn. The cases I never open still look pristine.
BCW makes snap-tight cases that claim scratch-resistant coating. I've used these for about 18 months and they do seem to hold up better than the magnetic cases. The coating is some kind of hard surface treatment-couldn't find specific info on what it is, but the cases are marketed as "premium grade" and cost about $6-7 each which is middle-range pricing.
Glass cases would solve the scratching problem but they're way more expensive and heavier. Ultra Pro made some glass card holders a few years back that were in the $25-30 range. They looked great but were impractical for storing more than a handful of cards. Most collectors can't spend $30 per card on display cases.

What about long-term storage, like 20+ years
This is where data gets scarce because modern sports card collecting only really took off in the late 1980s-early 1990s, and acrylic cases weren't widely used until the 2000s. So we don't have great empirical data on how cards stored in acrylic cases for 30+ years hold up compared to other methods.
Archival storage research from library science suggests PMMA acrylic is reasonably stable and won't degrade under normal storage conditions. The material doesn't off-gas acids or other compounds that would damage paper. That's good. PVC on the other hand (which is what cheap card pages and some old holders were made from) degrades and releases hydrochloric acid as it breaks down. This is why you see vintage cards with PVC staining-the storage material itself damaged the cards.
Toploaders are also PVC-free now (they're PET or polypropylene usually) but older ones from the 90s were PVC. If you have cards in really old toploaders it's worth checking and replacing them. The degraded PVC has a distinctive smell, kind of chemically and unpleasant.
The seals on acrylic cases degrade over time too. Magnetic cases use adhesive-backed magnets and that adhesive breaks down. I have cases from 2015-2016 where the magnets are starting to peel off. The case still closes but it doesn't seal as tightly. Snap-tight cases rely on physical clips which seem more durable but I've had clips break on cheaper cases after repeated opening.
Grading companies use ultrasonic welding on slabs
PSA, BGS, CGC-all the major grading companies seal their slabs with ultrasonic welding which creates a permanent bond. The cards inside are basically sealed off from the environment. This is better protection than any acrylic case you can buy and open yourself. But you're paying $25-150+ per card for grading depending on service level and turnaround time, plus the card is locked away permanently unless you crack the slab.
There's debate in the collecting community about whether grading is worth it for protection alone versus just for authentication and resale value. A PSA 10 sells for more than a raw card in equivalent condition, but if you never plan to sell, is the grading fee worth the protection? Probably not for most cards under $500 value.
I have about 30 cards submitted for grading over the years. The protection is nice, the slabs are durable, but you can't really examine the card closely or enjoy it the same way as a raw card in a case you can open. Trade-offs.
Alternatives that work better for specific situations
For bulk storage of lower-value cards, those cardboard storage boxes with penny sleeves are fine. A 3200-count box costs maybe $8-12 and penny sleeves are like $2-3 per 100. Way more economical than individual acrylic cases and sufficient protection for cards worth under $10-20 each.
Team bags (those resealable plastic bags) are underrated for grouped sets or cards you want to keep together. They don't provide crush resistance like rigid cases but they seal against dust and moisture better than most acrylic cases. I use team bags inside storage boxes as an extra layer of protection.
Binder storage works fine if you use high-quality pages and don't overfill the pockets. The risk is the binder getting dropped or stored improperly where the cards bear weight from other objects. I've seen cards develop ring indentations from binder rings pressing through pages over time when the binder was stored upright on a shelf. This was with ultra pro platinum pages which are supposed to be archival quality (polypropylene, acid-free, PVC-free).
Some collectors use safety deposit boxes at banks for extremely valuable cards. Seems excessive but if you own cards worth $10,000+ each it might make sense. The environmental controls in bank vaults are pretty good-stable temperature and humidity year-round. Insurance becomes complicated though because you need to specifically insure collectibles which most homeowner's policies don't cover adequately.
The market is flooded with mediocre products
There's probably 50+ brands making acrylic card cases now, most of them are white-label products from the same few factories in China. The cases look identical because they're literally the same molds and materials, just different branding printed on them. This makes quality control a gamble.
I've bought "premium" cases from companies I'd never heard of for $7-8 each that turned out to be the exact same cases as the $3 generic ones, just marked up. The acrylic quality, fitment, everything was identical. Learned to stick with known brands after that-Ultra Pro, BCW, Pro-Mold. They're not perfect but at least there's some consistency.
The magnetic closure cases have gotten cheap enough that they're almost the default now. You can get them for $4-5 each in multipacks. This has basically killed the snap-tight market for single-card storage. Magnetic cases are just easier to use and feel more premium even though the actual protection is probably equivalent.
Recessed cases for thick cards are still expensive though. A proper holder for a 100pt+ card runs $10-15 and they're not widely available. This creates problems for patch cards and other premium inserts that don't fit in standard cases. You end up with this awkward situation where a $200 card is sitting in a $3 case that doesn't fit properly because the right case costs $15 and you can't find it locally.

What I actually use for my collection
Cards under $50: penny sleeve + toploader, stored in cardboard boxes with dividers. Works fine, cheap, easy to organize and access.
Cards $50-300: magnetic acrylic one-touch holders, stored upright in a case. Mix of Ultra Pro and BCW depending what was available when I bought them. These go in a climate-controlled room, not a garage or basement.
Cards over $300: submitted for grading if I think they'll grade well, otherwise magnetic holders with extra insurance documentation (photos, receipts, etc.). The graded slabs go in a safe with desiccant.
Vintage cards (pre-1980): all sleeved and in toploaders, some graded. The graded ones are in the safe, the others are in a sealed container with silica gel that I actually check and replace every 6 months. These cards are more susceptible to humidity damage because the cardstock is different from modern cards.
This system evolved over like 10 years of making mistakes and learning what works. Started with everything in penny sleeves in a shoebox (bad idea), then tried binder storage (cards got damaged), then switched to mostly toploaders, and finally added acrylic cases for the better cards once the prices came down.
So do acrylic card boxes prevent damage? Yeah, mostly. They stop physical damage, reduce UV exposure if you get the right ones, and make storage more organized. They don't prevent humidity issues, they scratch easily if you handle them a lot, and cheap cases aren't much better than toploaders. For cards worth protecting with acrylic cases (probably $30+ value minimum), buy decent quality cases from real brands and store them in a good environment. The case is only part of the protection equation.

